- PII
- S30345103S0016675825010033-1
- DOI
- 10.7868/S3034510325010033
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 61 / Issue number 1
- Pages
- 38-44
- Abstract
- The paper discusses a promising herbal preparation – thymoquinone, a component of black cumin (Nigella sativa), studied in experimental animals (mice, rats) in many pathologies, characterized by a positive effect and lack of toxic effect. The drug has been studied in a wide range of doses for injection and oral administration. Thymoquinone has antimicrobial, antiviral, anti-inflammatory, and radioprotective properties. The main damaging component of ionizing radiation is oxidative stress. For this reason, radioprotectors have recently been evaluated based on the drug’s ability to reduce oxidative stress. As markers of oxidative stress, we used parameters of changes in the expression of nuclear and mitochondrial DNA genes that perform essential functions in the cell. C57Bl/6 mice were administered thymoquinone (10 mg/kg) after 30 min. irradiation was performed (6 Gy). After 6 and 24 hours, gene expression in brain and spleen cells was studied using real-time PCR. It was shown that the activity of nuclear genes increased after exposure to radiation, but was normalized if thymoquinone was administered to mice 30 minutes before irradiation. Mitochondrial genes were also modified to target the activity of control cells. The test results show that thymoquinone has protective properties and may be promising as a radioprotector.
- Keywords
- радиопротекторы тимохинон ядерные и митохондриальные гены
- Date of publication
- 01.01.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 32
References
- 1. Ильин Л.А. Медицинские аспекты противодействия радиологическому и ядерному терроризму. М.: Наука, 2018. 392 с.
- 2. Ушаков И.Б. Космос, радиация, человек. М.: Научтехлитиздат, 2021. 352 с.
- 3. Dogru S., Taysi S., Yucel A. Effect soft hymoquinone in the lungs of rats against radiation-induced oxidative stress // Eur. Rev. Med. and Pharmacol. Sci. 2024. V. 28. № 1. P. 191–198. https://doi.org/10.26355/eurrev_202401_34904
- 4. Михайлов В.Ф., Засухина Г.Д. Новый подход к стимуляции защитных систем организма малыми дозами радиации // Успехи соврем. биол. 2020. Т. 140. № 3. С. 244–252. https://doi.org/10.31857/S0042132420030060
- 5. Altay H., Demir E., Binici H. et al. Radioprotective effects of propolis and caffeic acid phenethyl ester on the tong-tissues // Eur. J. Theоr. 2020. V. 26. P. 202–207. https://doi.org/10.5152/eurjther.2020.19047
- 6. Taysi S., Algburi F., Mohammed Z. et al. Thymoquinone: А review on its pharmacological importance and its associftion with oxidative stress, COVID 19 and radiotherapy // Mini Rev. Med. Chem. 2022. V. 22. № 14. P. 1874–1875 https://doi.org/10.2174/1389557522666220104151225
- 7. Sadeghi E., Inenshahidi M., Hosseinzadeh H. Molecular mechanisms and signaling pathways of black cumin (Nigella sativa) and its active constituent, thymoquinone: А review // Mol. Biol. Rep. 2023. V. 50. P. 5439–5454. https://doi.org/10.1007/s11033-023-08363-y
- 8. Tiwari G., Cupta M., Devhare L., Tiwari R. Therapeutic and phytochemical properties of thymoquinone derived from Nigella sativa // Curr. Drug Res. Rev. 2023. https://doi.org/10.2174/2589977515666230811092410
- 9. Demir Е., Taysi S., Ulisal H. et al. Nigella sativa oil and thymoquinone reduce oxidative stress in the brain tissue of rats exposed to total head irradiation // Int. J. Radiat. Biol. 2020. V. 96. № 2. P. 228–235. https://doi.org/10.1080/09553002.2020.1683636
- 10. Akyuz M., Taysi S., Baysal E. et al. Radioprotective effect of thymoquinone on salvary gland of rats exposed to total cranial irradiation // Head Neck. 2017. V. 39. № 10. P. 2027–2035. https://doi.org/10.1002/hed.24861
- 11. Koc M., Deniz C., Eryilmaz M. et al. Radioprotective effects of melatonine and thymoquinone on liver, hfrotid gland, brain, and testis of rats exposed to total body irradiation // Turk. J. Med. Sci. 2023. V. 53. P. 902–908. https://doi.org/10.55730/1300-0144.5654
- 12. Ahmed S., Bakz M. Will Nigella sativa oil protect parotil glands of rats against cranium gamma radiation? Histological and immunohistochemical evaluation // BMC Complement Med. Ther. 2024. V. 24. P. 111. https://doi.org/10.1186/s12906-024-04410-8
- 13. Abdullaev S., Gubina N., Bulanova T. et al. Assessment of nuclear and mitochondrial DNA, expression of mitochondria-related genes in different brain regions in rats after whole-body X-ray irradiation // Int. J. Mol. Sci. 2020. V. 21. https://doi.org/10.3390/ijms21041196
- 14. Abdullaev S.A., Glukhov S.I., Gaziev A.I. Radioprotective and radiomitigative effects of melatonin in tissues with different proliferative activity // Antioxidants (Basel). 2021. V. 10. https://doi.org/10.3390/antiox10121885
- 15. Газиев А.И. Пути сохранения целостности митохондриальной ДНК и функций митохондрий в клетках, подвергшихся воздействию ионизирующей радиации // Радиац. биол. Радиоэкология. 2013. Т. 53. № 2. С. 117–136.
- 16. Михайлов В.Ф., Салеева Д.В., Рождественский Л.М. и др. Активность генов и некодирующих РНК как подход к определению ранних биомаркеров радиоиндуцированного опухолеобразования у мышей // Генетика. 2021. Т. 57. № 10. С. 1131–1140. https://doi.org/10.31857/S0016675821100076
- 17. Long G., Chen H., Wu M. et al. Antianemia drug roxadustat (FG-4592) protects against doxorubicin-induced cardiotoxicity by targeting antiapoptotic and antioxidative pathways // Front. Pharmacol. 2020. V. 11. https://doi.org/10.3389/fphar.2020.01191
- 18. Wang H., Yu W., Wang Y. et al. P53 contributes to cardiovascular diseases via mitochondria dysfunction: A new paradigm // Free Radic. Biol. Med. 2023. V. 208. P. 846–858. https://doi.org/10.1016/j.freeradbiomed.2023.09.036
- 19. Alsanosi S., Sheikh R., Sonbul S. et al. The potential role of Nigella sativa seed oil as epigenetic therapy of cancer // Molecules. 2022. V. 27. https://doi.org/10.3390/molecules27092779
- 20. Kaleem M., Kayali A., Sheikh R. et al. In vitro and in vivo preventive effects of thymoquinone against breast cancer-role of DNMT1 // Molecules. 2024. V. 29. P. 434.
- 21. Салеева Д.В., Раева Н.Ф., Абдуллаев С.А. и др. Профилактический и терапевтический потенциал тимохинона при ряде патологий человека на основе определения активации клеточных компонентов, осуществляющих защитные функции по активности генов и некодирующих РНК // Госпитальная медицина: наука и практика, 2023. T. 6. № 2. C. 27–36. https://doi.org/10.34852/GM3CVKG.2023.75.38.015
